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anti il 15 detection  (R&D Systems)


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    R&D Systems anti il 15 detection
    Anti Il 15 Detection, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/biotinylated+anti+human+il+15+antibody/Human%2FPrimate+IL-15+Biotinylated+Antibody/us12371472-1451-16-19
    Average 93 stars, based on 14 article reviews
    anti il 15 detection - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Expressing:

    Article Title: Multimeric IL-15 soluble fusion molecules and methods of making and using same
    Article Snippet: .. The puromycin and G418 resistant cells were expanded and the T2 fusion protein complex expressing cells were subcloned three times by the limiting dilution and production cell lines were screened based on the level of soluble fusion protein secreted into the culture media using a huIgG1/huIL15-specific ELISA with a capture antibody, anti-human IgG antibody (Jackson ImmunoResearch), and a detection antibody, biotinylated anti-human IL-15 antibody (BAM 247, R&D Systems). ..

    Article Title: Multimeric IL-15 soluble fusion molecules and methods of making and using same
    Article Snippet: The cells were selected with 4 mg/mL of G418 (Invitrogen) one day after the transfection. .. The G418 resistant cells were expanded and TCR/IL15 fusion protein expressing cells were subcloned three times by the limiting dilution and production cell lines were screened based on the level of soluble fusion protein secreted into the culture media by TCR and huIL15-specific ELISA with a capture antibody, anti-human TCR Cβ antibody (BF1), and a detection antibody, biotinylated anti-human IL-15 antibody (BAM 247, R&D Systems) described previously (see WO2008143794). ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Multimeric IL-15 soluble fusion molecules and methods of making and using same
    Article Snippet: .. The puromycin and G418 resistant cells were expanded and the T2 fusion protein complex expressing cells were subcloned three times by the limiting dilution and production cell lines were screened based on the level of soluble fusion protein secreted into the culture media using a huIgG1/huIL15-specific ELISA with a capture antibody, anti-human IgG antibody (Jackson ImmunoResearch), and a detection antibody, biotinylated anti-human IL-15 antibody (BAM 247, R&D Systems). ..

    Article Title: Multimeric IL-15 soluble fusion molecules and methods of making and using same
    Article Snippet: The cells were selected with 4 mg/mL of G418 (Invitrogen) one day after the transfection. .. The G418 resistant cells were expanded and TCR/IL15 fusion protein expressing cells were subcloned three times by the limiting dilution and production cell lines were screened based on the level of soluble fusion protein secreted into the culture media by TCR and huIL15-specific ELISA with a capture antibody, anti-human TCR Cβ antibody (BF1), and a detection antibody, biotinylated anti-human IL-15 antibody (BAM 247, R&D Systems) described previously (see WO2008143794). ..

    other:

    Article Title: Multi-chain chimeric polypeptides and uses thereof
    Article Snippet: After 24 hrs of incubation at 37° C., 40 of induced HEK-Blue TGFβ cell supernatant was added to 160 μL pre-warmed QUANTI-Blue (Invivogen) in a flat-bottom 96-well plate, and incubated at 37° C. for 1-3 hrs.

    Article Title: Methods of treating aging-related disorders
    Article Snippet: The anti-TF antibody affinity column was connected to a GE Healthcare AKTA Avant system.



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